IVF Treatment in Pune Delivered with Care, Expertise & Personal Touch

The next stage in the IVF process is referred to as ovarian stimulation or controlled ovarian stimulation (COS). To stimulate the ovaries and encourage the growth of multiple mature eggs, you will receive a prescription for fertility medications, usually in the form of injectables. The medication protocol will be personalized to suit your individual requirements and closely monitored through regular ultrasound scans and hormone level assessments to ensure optimal results.

Before egg retrieval, you will be given medicine to keep you from feeling pain and help you relax. Once the eggs have reached the desired level of maturity, a scheduled egg retrieval procedure is conducted. This procedure generally takes place with the guidance of ultrasound and under light anesthesia.
A thin needle is inserted through the vaginal wall into the ovaries, and the follicular fluid containing the eggs is gently aspirated. The procedure typically lasts about 20 minutes, after which the eggs are carefully transferred to the embryology laboratory. Eggs that appear mature and healthy will be mixed with sperm to attempt to create embryos. After the procedure, you may have cramping and feelings of pressure or fullness.

Generally, on the same day as the egg retrieval, a fresh semen sample is obtained from your spouse to proceed with the egg harvest. However, a previously frozen sample can be used if it is difficult to collect a fresh sample or if your spouse is unavailable on that day. The sperm and eggs will then be combined in a petri dish by a skilled and experienced technician.

Conventional IVF and Intracytoplasmic Sperm Injection (ICSI) are the two primary methods of fertilization used in IVF. Conventional IVF involves combining the retrieved eggs and collected sperm in a culture dish to allow natural fertilization. Meanwhile, ICSI may be recommended if the quality of the sperm sample is not good enough to do IVF or if there is a male infertility issue. This procedure involves injecting live sperm directly into each mature egg to facilitate fertilization.

Your embryo must prevail over hurdles to become an embryo suitable for transfer to your uterus. Only 50% (on average) of fertilized embryos advance to the blastocyst stage, an ideal stage for transfer to your uterus. Embryos suitable for transfer will be frozen on the fifth- or sixth-day post-fertilization for future embryo transfers.

Preimplantation genetic testing (PGT) identifies genetic abnormalities (in embryos) created with IVF. It involves the biopsy of one or a few cells from each embryo for genetic testing. Embryos predicted to be free of chromosomal abnormalities or specific genetic conditions are selected. The test will provide you with the opportunity to minimize the chance of a genetic disease in your future children before pregnancy.

The most appropriate embryos are chosen for transfer based on their quality, the quantity of available embryos, and individual circumstances. Embryo transfer generally does not require anesthesia. A thin catheter housing the embryos is delicately inserted through the cervix into the uterus. Subsequently, the embryos are gently released into the uterine cavity.

Once the embryos have been transferred into the womb, you will be recommended to take a brief period of rest before resuming regular activities. A pregnancy test is typically conducted after around two weeks to find out if the treatment has worked. If you do become pregnant, further monitoring and assistance will be provided to check if things are progressing as expected.